Review



human peripheral nerve pericyte  (ScienCell)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    ScienCell human peripheral nerve pericyte
    (A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
    Human Peripheral Nerve Pericyte, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+nerve+pericyte/human+peripheral+nerve+pericyte/pmc12092546-78-7-45
    Average 90 stars, based on 1 article reviews
    human peripheral nerve pericyte - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome"

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome

    Journal: Neurology® Neuroimmunology & Neuroinflammation

    doi: 10.1212/NXI.0000000000200405

    (A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
    Figure Legend Snippet: (A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.

    Techniques Used: Immunostaining, Expressing, Incubation, Purification, Permeability, In Vitro

    Related Articles

    Suspension:

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome
    Article Snippet: Three-lane organoplates with 400 μm × 220 μm (w × h) channels (Mimetas BV) were used to create microfluidic in vitro BNB coculture models. .. After loading the cell suspension with human peripheral nerve pericyte (2.5 × 10 6 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function. ..

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome
    Article Snippet: .. Three-lane organoplates with 400 μm × 220 μm (w × h) channels (Mimetas BV) were used to create microfluidic in vitro BNB coculture models.18,19 After loading the cell suspension with human peripheral nerve pericyte (2.5 × 106 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function. ..

    Cell Culture:

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome
    Article Snippet: Three-lane organoplates with 400 μm × 220 μm (w × h) channels (Mimetas BV) were used to create microfluidic in vitro BNB coculture models. .. After loading the cell suspension with human peripheral nerve pericyte (2.5 × 10 6 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function. ..

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome
    Article Snippet: .. Three-lane organoplates with 400 μm × 220 μm (w × h) channels (Mimetas BV) were used to create microfluidic in vitro BNB coculture models.18,19 After loading the cell suspension with human peripheral nerve pericyte (2.5 × 106 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function. ..

    In Vitro:

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome
    Article Snippet: .. Three-lane organoplates with 400 μm × 220 μm (w × h) channels (Mimetas BV) were used to create microfluidic in vitro BNB coculture models.18,19 After loading the cell suspension with human peripheral nerve pericyte (2.5 × 106 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function. ..



    Similar Products

    peripheral nerve pericyte line  (PromoCell)
    95
    PromoCell peripheral nerve pericyte line
    Peripheral Nerve Pericyte Line, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+nerve+pericyte/Human+Pericytes+from+Placenta/pm40381746-131-14-9
    Average 95 stars, based on 1 article reviews
    peripheral nerve pericyte line - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    human peripheral nerve pericyte  (ScienCell)
    90
    ScienCell human peripheral nerve pericyte
    (A) Immunostaining of human <t>peripheral</t> nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and <t>pericytes.</t> (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.
    Human Peripheral Nerve Pericyte, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+nerve+pericyte/human+peripheral+nerve+pericyte/pmc12092546-78-7-45
    Average 90 stars, based on 1 article reviews
    human peripheral nerve pericyte - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.

    Journal: Neurology® Neuroimmunology & Neuroinflammation

    Article Title: Small Nuclear Ribonucleoprotein Autoantibody Associated With Blood-Nerve Barrier Breakdown in Guillain-Barré Syndrome

    doi: 10.1212/NXI.0000000000200405

    Figure Lengend Snippet: (A) Immunostaining of human peripheral nerve microvascular endothelial cells (PnMECs) for snRNPs. Nuclear snRNP expression in monolayer PnMECs decreased after incubation with GBS-IgG with snRNP antibodies or anti-snRNP monoclonal antibody compared with after cont-IgG or cont-Ab. GBS-IgG: IgG purified from serum samples of 20 patients with U1-snRNP autoantibody–positive GBS; cont-IgG: IgG purified from serum samples of 10 healthy controls; RNP antibody: commercial snRNP monoclonal antibody; cont-Ab: commercial anti-rabbit IgG. Scale bar, 50 μm. (B) Incubation of GBS-IgG with snRNP antibodies or anti-snRNP antibody caused a significant increase in 10-kDa dextran permeability, compared with that with cont-IgG or cont-Ab, using a monolayer coculture model consisting of FH-BNB cells and pericytes. (C) GBS-IgG with anti-snRNP antibody induced a significant increase in the permeability of FH-BNB compared with cont-IgG, GBS-IgG without anti-snRNP antibody, or GBS-IgG after the reduction of U1-snRNP antibody from GBS-IgG with U1-snRNP antibody. (D) Incubation with GBS-IgG or snRNP antibodies leads to a decrease in claudin-5 and an increase in 10-kDa dextran permeability compared with cont-IgG using the spheroid model, which consists of both FH-BNB cells and pericytes (Left). Scale bar, 50 μm. Permeability of 20-kDa dextran increased after exposure to GBS-IgG or snRNP antibodies compared with cont-IgG using a microfluidic in vitro BNB coculture model (Right). Scale bar, 100 μm. BNB = blood-nerve barrier; U1-snRNP = U1-small nuclear ribonucleoprotein.

    Article Snippet: After loading the cell suspension with human peripheral nerve pericyte (2.5 × 10 6 cells/mL) in collagen I gel (2 μL) into the middle channel, PnMECs were seeded and cultured in the top channel to establish a BNB microfluidic coculture model. CB medium and fresh Sciencell pericyte medium were used to assess their influence on barrier function.

    Techniques: Immunostaining, Expressing, Incubation, Purification, Permeability, In Vitro